Q
is a basic dye and is a negative stain.
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A basic dye, by definition, is a positively charged dye that binds to negatively charged molecules, commonly used in microbiology to stain the cell walls of bacteria, which are negatively charged. This characteristic makes basic dyes ideal for direct staining where the dye interacts directly with the specimen material. On the other hand, a negative stain does not directly stain the specimen itself but instead stains the space around it, producing a silhouette effect. This type of staining is particularly useful for visualizing cell shapes and sizes without altering or penetrating cells, as the negatively charged dye particles are repelled by the negatively charged cell walls, thus remaining unstained. Examples of basic dyes include methylene blue and crystal violet, while examples of negative stains include nigrosin and India ink. Using the contrasting properties of basic dyes and negative stains can provide a comprehensive understanding of cellular structures in microscopic studies.
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