Q
how to coat tubes in fbs
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Coating tubes with Fetal Bovine Serum (FBS) is a method often used in biological and medical research to promote cell attachment to the tube surface. FBS contains various growth factors and proteins that enhance cell adhesion, making it an ideal coating solution. To coat tubes with FBS, follow these steps: First, ensure tubes are clean and sterile. Then, add enough FBS to cover the surface of the tube, normally a few milliliters depending on the tube size. Incubate the tubes at room temperature for 1-2 hours or at 4°C overnight to allow protein adsorption. After incubation, aspirate the excess FBS and rinse the tubes with phosphate-buffered saline (PBS) or sterile water to remove any unbound serum. Tubes are now ready for use and should be kept sterile until used. It’s essential to use sterile techniques to prevent contamination.
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