Q
what are the dyes added in emb agar
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In EMB agar, which stands for Eosin Methylene Blue agar, the dyes eosin Y and methylene blue are added. These dyes serve multiple purposes: they are selective agents that inhibit the growth of Gram-positive bacteria, allowing for the preferential growth of Gram-negative bacteria. Additionally, they act as indicators, providing a color reaction in the presence of lactose fermenters. E. coli, for instance, produces a metallic green sheen on EMB agar due to its acid production from lactose fermentation. Non-lactose fermenters, on the other hand, either grow as colorless colonies or take on the color of the dyes. This makes EMB agar highly useful in the differential identification and isolation of enteric bacteria, particularly in the field of microbiology where the differentiation between lactose fermenting and non-fermenting organisms is essential.
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