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Q
what kind of dyes do lymphocytes pick up
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In the field of hematology and immunology, lymphocytes, which are a type of white blood cell pivotal in the immune system, can be stained using various dyes for identification and analysis under a microscope. Commonly, Romanowsky-type dyes, which include Wright, Wright-Giemsa, and Leishman stains, are used. These dyes impart distinct coloration to lymphocytes, enhancing the visibility of their characteristic large nucleus which stains dark purple, and a small rim of cytoplasm which appears in a lighter blue or pink hue, depending on the specific dye and its pH. Additionally, immunofluorescence techniques employing fluorescently labeled antibodies can selectively bind to lymphocytes based on specific markers or antigens, allowing for their identification and study of specific subpopulations or cell states. These methodologies are crucial in diagnostics and research, providing insights into immune responses and diseases like lymphomas.
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