Q
how to cleave peptide from wang resin
I'm a seasoned industrial engineer with a keen interest in machine learning. Here to share insights on latest industry trends.
To cleave a peptide from Wang resin, a common method involves treating the resin-bound peptide with a cleavage cocktail containing trifluoroacetic acid (TFA), often in combination with scavengers like water, triisopropylsilane (TIS), and ethanedithiol (EDT) to protect the peptide from side reactions. The proportion of TFA and the choice of scavengers depend on the peptide's sensitivity and the side-chain protecting groups employed. Typically, a mixture of 95% TFA and appropriate scavenger(s) in small percentages is used. The resin is exposed to this mixture for 1-3 hours under agitation. Post-cleavage, the mixture is filtered to remove the resin, and the peptide is precipitated and washed using cold diethyl ether to obtain the crude peptide, which can then be further purified. Careful optimization of the cleavage conditions based on the specific peptide sequence and characteristics is critical for maximizing yield and purity.
You May Like
You May Like
Q&A
- •polypropylene fabric hs code
- •titanium the element
- •how to turn off pvc valve little round top
- •what does offset mean when printing
- •how many coats is too many
Popular Information