Q
how to insert new amino acid into peptide
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Inserting a new amino acid into a peptide involves highly specialized chemical or enzymatic synthesis techniques. Chemically, solid-phase peptide synthesis (SPPS) is a common method, where amino acids are added sequentially to a growing peptide chain anchored on a solid resin. Each addition requires deprotection steps to expose the functional groups for the next amino acid attachment, followed by coupling reactions to form peptide bonds. Enzymatically, methods like expressed protein ligation or intein-mediated splicing allow for the insertion of amino acids into peptides or proteins by creating a new peptide bond between a peptide and an added amino acid or peptide fragment. These approaches require precise control over reaction conditions and are important tools in biochemistry and molecular biology for studying protein function and for designing novel peptides with desired properties.
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