Q
why are amino acid absorbances linearly independent
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Amino acid absorbances are linearly independent because each amino acid has a unique structure, which results in specific absorbance properties at different wavelengths, primarily due to the distinct side chains. This specificity is notably observed in the UV spectrum, where peptides and proteins absorb light due to the presence of aromatic amino acids like tyrosine, tryptophan, and phenylalanine. Since the absorption of light is a physical property influenced by the molecular structure, each amino acid's absorption profile is determined by its unique side chain, leading to their linear independence. This is applied in techniques like UV/Vis spectroscopy for protein quantification and analysis, leveraging the differences in absorbance patterns to distinguish and quantify amino acids and proteins.
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